CD166 antibody (Extracellular Domain) (Biotin)

Catalog No. ABIN602948

The Goat Polyclonal anti-CD166 antibody has been validated for WB and ELISA. It is suitable to detect CD166 in samples from Human.

Quick Overview for CD166 antibody (Extracellular Domain) (Biotin) (ABIN602948)

Target: CD166 (ALCAM) (Activated Leukocyte Cell Adhesion Molecule (ALCAM))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This CD166 antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA

Product Details anti-CD166 Antibody (Biotin)

Binding Specificity: Extracellular Domain

Specificity: Recognizes human ALCAM. Less than 0.2 % cross-reactivity with rmALCAM, rhICAM-2, rhICAM-3, rhE-Selectin and rhP-Selectin is observed.

Purification: Immunoaffinity purified

Sterility: Sterile filtered

Immunogen: NS0-derived recombinant human ALCAM extracellular domain.

Isotype: IgG

Application Details

Application Notes: Approved: ELISA, WB

Usage: Suitable for use in Western Blot. Using plates coated with 100 μL/well of the capture antibody, in combination with 100 μL/well of the detection antibody at 50 ng/mL, an ELISA for sample volumes of 100 μL can be obtained. To arrive at the optimal dose range for this ELISA, set up a two-fold dilution series of the protein standard starting with 4 ng/mL. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile TBS,  pH 7.3, 0.1 % BSA.

Concentration: Lot specific

Buffer: Lyophilized from 0.2 um sterile-filtered solution in PBS, 50 μg BSA

Storage: 4 °C,-20 °C

Storage Comment: Lyophilized powder may be stored at 4°C for short-term only. Reconstituted product is stable for 1 year at -20°C.

Target Details for CD166

Target: CD166 (ALCAM) (Activated Leukocyte Cell Adhesion Molecule (ALCAM))

Alternative Name: ALCAM / CD166

Background: Name/Gene ID: ALCAM

Synonyms: ALCAM, CD166 antigen, MEMD, CD166

Gene ID: 214

UniProt: Q13740